The key to successful performance of BI lies in the design of simple and reliable approaches to:
- Bead metering and transport
- Bead packing and perfusion
- Monitoring of a change of bead optical properties
While for Bead Injection Chromatography (BIC) it is sufficient to meet the first two requirements, Bead Injection Spectroscopy (BIS) is more difficult to perform, since the flow cells for monitoring of absorbance, fluorescence or chemiluminescence have to be carefully constructed, and the volume of bead suspension and packing density of the column must be very precisely controlled.
Over the years, an astonishing variety of approaches have been designed for bead handling and capture (Chandler 2000), yet ultimately a simple approach as shown in 3.2.6. and 3.2.7., has been designed that exploits the versatility of programmable flow and combines precisely tuned flow rates and flow reversals for bead transport, packing and perfusion.
D.P.Chandler, F.J. Brockman, D.A. Holman, J.W.Grate & C.J.Bruckner-Lea, TRAC, 19, 314 (2000).
Key Operations
3.2.3.
If anything can go wrong, it will.
SNAFU
